A Novel Chemical Compound for Inhibition of SARS Coronavirus Helicase.
Identifieur interne : 000E83 ( Main/Exploration ); précédent : 000E82; suivant : 000E84A Novel Chemical Compound for Inhibition of SARS Coronavirus Helicase.
Auteurs : Jin-Moo Lee [Corée du Sud] ; Jin-Beom Cho [Corée du Sud] ; Hee-Chul Ahn [Corée du Sud] ; Woong Jung [Corée du Sud] ; Yong-Joo Jeong [Corée du Sud]Source :
- Journal of microbiology and biotechnology [ 1738-8872 ] ; 2017.
Descripteurs français
- KwdFr :
- ADN (antagonistes et inhibiteurs), Adénosine triphosphate, Antienzymes (), Antienzymes (isolement et purification), Antienzymes (pharmacologie), Antiviraux (), Antiviraux (antagonistes et inhibiteurs), Antiviraux (isolement et purification), Concentration inhibitrice 50, Helicase (), Humains, Hydrolyse, Lignée cellulaire (), Survie cellulaire (), Virus du SRAS (), Virus du SRAS (enzymologie).
- MESH :
- antagonistes et inhibiteurs : ADN, Antiviraux.
- enzymologie : Virus du SRAS.
- isolement et purification : Antienzymes, Antiviraux.
- pharmacologie : Antienzymes.
- Adénosine triphosphate, Antienzymes, Antiviraux, Concentration inhibitrice 50, Helicase, Humains, Hydrolyse, Lignée cellulaire, Survie cellulaire, Virus du SRAS.
English descriptors
- KwdEn :
- Adenosine Triphosphate, Antiviral Agents (antagonists & inhibitors), Antiviral Agents (chemistry), Antiviral Agents (isolation & purification), Cell Line (drug effects), Cell Survival (drug effects), DNA (antagonists & inhibitors), DNA Helicases (drug effects), Enzyme Inhibitors (chemistry), Enzyme Inhibitors (isolation & purification), Enzyme Inhibitors (pharmacology), Humans, Hydrolysis, Inhibitory Concentration 50, SARS Virus (drug effects), SARS Virus (enzymology).
- MESH :
- chemical , antagonists & inhibitors : Antiviral Agents, DNA.
- chemical , chemistry : Antiviral Agents, Enzyme Inhibitors.
- chemical , drug effects : DNA Helicases.
- chemical , isolation & purification : Antiviral Agents, Enzyme Inhibitors.
- chemical , pharmacology : Enzyme Inhibitors.
- chemical : Adenosine Triphosphate.
- drug effects : Cell Line, Cell Survival, SARS Virus.
- enzymology : SARS Virus.
- Humans, Hydrolysis, Inhibitory Concentration 50.
Abstract
We have discovered a novel chemical compound, (E)-3-(furan-2-yl)-N-(4-sulfamoylphenyl) acrylamide, that suppresses the enzymatic activities of SARS coronavirus helicase. To determine the inhibitory effect, ATP hydrolysis and double-stranded DNA unwinding assays were performed in the presence of various concentrations of the compound. Through these assays, we obtained IC50 values of 2.09 ± 0.30 µM (ATP hydrolysis) and 13.2 ± 0.9 µM (DNA unwinding), respectively. Moreover, we found that the compound did not have any significant cytotoxicity when 40 µM of it was used. Our results showed that the compound might be useful to be developed as an inhibitor against SARS coronavirus.
DOI: 10.4014/jmb.1707.07073
PubMed: 28910865
Affiliations:
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Le document en format XML
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<term>Cell Line (drug effects)</term>
<term>Cell Survival (drug effects)</term>
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<term>Enzyme Inhibitors (chemistry)</term>
<term>Enzyme Inhibitors (isolation & purification)</term>
<term>Enzyme Inhibitors (pharmacology)</term>
<term>Humans</term>
<term>Hydrolysis</term>
<term>Inhibitory Concentration 50</term>
<term>SARS Virus (drug effects)</term>
<term>SARS Virus (enzymology)</term>
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<term>Adénosine triphosphate</term>
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<term>Antienzymes (pharmacologie)</term>
<term>Antiviraux ()</term>
<term>Antiviraux (antagonistes et inhibiteurs)</term>
<term>Antiviraux (isolement et purification)</term>
<term>Concentration inhibitrice 50</term>
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<term>Humains</term>
<term>Hydrolyse</term>
<term>Lignée cellulaire ()</term>
<term>Survie cellulaire ()</term>
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<front><div type="abstract" xml:lang="en">We have discovered a novel chemical compound, (E)-3-(furan-2-yl)-<i>N</i>
-(4-sulfamoylphenyl) acrylamide, that suppresses the enzymatic activities of SARS coronavirus helicase. To determine the inhibitory effect, ATP hydrolysis and double-stranded DNA unwinding assays were performed in the presence of various concentrations of the compound. Through these assays, we obtained IC<sub>50</sub>
values of 2.09 ± 0.30 µM (ATP hydrolysis) and 13.2 ± 0.9 µM (DNA unwinding), respectively. Moreover, we found that the compound did not have any significant cytotoxicity when 40 µM of it was used. Our results showed that the compound might be useful to be developed as an inhibitor against SARS coronavirus.</div>
</front>
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<tree><country name="Corée du Sud"><region name="Région capitale de Séoul"><name sortKey="Lee, Jin Moo" sort="Lee, Jin Moo" uniqKey="Lee J" first="Jin-Moo" last="Lee">Jin-Moo Lee</name>
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<name sortKey="Ahn, Hee Chul" sort="Ahn, Hee Chul" uniqKey="Ahn H" first="Hee-Chul" last="Ahn">Hee-Chul Ahn</name>
<name sortKey="Cho, Jin Beom" sort="Cho, Jin Beom" uniqKey="Cho J" first="Jin-Beom" last="Cho">Jin-Beom Cho</name>
<name sortKey="Jeong, Yong Joo" sort="Jeong, Yong Joo" uniqKey="Jeong Y" first="Yong-Joo" last="Jeong">Yong-Joo Jeong</name>
<name sortKey="Jung, Woong" sort="Jung, Woong" uniqKey="Jung W" first="Woong" last="Jung">Woong Jung</name>
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